期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 39, 期 11, 页码 4145-4148出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.39.11.4145-4148.2001
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Quantitative real-time PCR was used to assay spirochetes in feeding ticks. Spirochetes in tick midguts increased sixfold, from 998 per tick before attachment to 5,884 at 48 h of attachment. Spirochetes in tick salivary glands increased > 17-fold, from 1.2 per salivary gland pair before feeding to 20.8 at 72 h postattachment. The period of the most rapid increase in the number of spirochetes in the salivary glands occurred from 48 to 60 h postattachment; this time period coincides with the maximal increase in transmission risk during nymphal tick feeding.
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