期刊
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
卷 126, 期 6, 页码 668-675出版社
AMER SOC HORTICULTURAL SCIENCE
DOI: 10.21273/JASHS.126.6.668
关键词
cultivar identification; phenetic similarities
类别
One hundred and thirteen olive (Olea europaea L.) accessions were characterized using randomly amplified polymorphic DNA (RAPD) markers. Forty-five polymorphic RAPD markers were obtained enabling us to distinguish 102 different RAPD profiles. The approximate estimation of the probability of obtaining the same RAPD profile for two different trees was between 6.75 x 10(-5) and 4.82 x 10(-14). A dendrogram was constructed using Ward's minimum variance algorithm based on chi-square distances. This led to a more clear-cut classification of profiles than the classical approach of unweighted pair group method with arithmetic average. Twenty-four clusters of RAPD profiles were shown in Ward's dendrogram. Reliability of the dendrogram structure was checked using variance analysis. RAPD data exhibited an acceptable resolving power for cultivar identification. A combination of three primers was proposed for rapid molecular identification of cultivars in collections and in nurseries.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据