期刊
AUTOPHAGY
卷 5, 期 5, 页码 690-698出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/auto.5.5.8494
关键词
GABARAP; Nix; Bnip3L; phage display; protein-protein-interaction
类别
资金
- International Helmholtz Research School BioSoft
- Deutsche Forschungsgemeinschaft (DFG)
- Deutscher Akademischer Austauschdienst (DAAD) [Wi1472/5]
Autophagy, a pathway primarily relevant for cell survival, and apoptosis, a process invariably leading to cell death, are the two main mechanisms of cellular self-destruction, which are essential in cell growth, neurodegeneration, tumor suppression, stress and immune response. Currently, a potential crosstalk between apoptosis and autophagy is subject to intensive investigations since recently some direct junctions became obvious. The respective protein-protein interaction network, however, remains to be elucidated in detail. The gamma-aminobutyric acid type A (GABA(A)) receptor-associated protein GABARAP belongs to a family of proteins implicated in intracellular transport events and was shown to be associated to autophagic processes. Using a phage display screening against the target protein GABARAP, we identified the proapoptotic protein Nix/Bnip3L to be a potential GABARAP ligand. In vitro binding studies, pull-down analysis, coimmunoprecipitation assays and colocalization studies confirmed a direct interaction of both proteins in mammalian cells.
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