期刊
BRAIN RESEARCH
卷 917, 期 2, 页码 139-146出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0006-8993(01)02794-9
关键词
GABA; glutamate; mossy fiber; kindling; epilepsy seizure; GAD
In the normal granule cells of the dentate gyrus glutamate, GABA and glutamic acid decarboxylase (GAD(67)) coexist. After kindled seizures, this enzyme is transiently overexpressed and simultaneous glutamatergic and GABAergic transmission in the mossy fiber projection occurs. Since this dual transmission is also seen after acutely-induced seizures, we decided to study the relationship between the expression of GAD(67) and the induction of simultaneous glutamatergic and GABAergie transmission by kindled or acutely induced seizures. We also explored whether kindling of the dentate gyrus in vitro, that does not induce epileptiform activity, could induce the expression of GAD(67). We confirm that kindling epilepsy induces the expression of GAD(67) in the dentate gyrus. Despite the emergence of GABAergic transmission in the mossy fiber projection after a single seizure, GAD(67) expression in the dentate gyrus appeared similar to controls, however, in the mossy fibers an enhanced immunostaining was evident. Interestingly, kindling the dentate gyrus in vitro induces a marked GAD(67) staining in the granule cells. Our results show that after the activity-dependent emergence of simultaneous glutamatergic and GABAergic transmission from the mossy fibers, GAD(67) is expressed in the mossy fibers and, upon long-lasting enduring stimulation periods, also in the dentate gyrus. Thus, this phenomenon does not depend on the presence of epileptic activity, but rather, on increased excitatory input onto the dentate gyrus, This can represent a protective mechanism that can sustain GABA synthesis in an activity-dependent manner. (C) 2001 Elsevier Science B.V. All rights reserved.
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