期刊
ANALYTICAL BIOCHEMISTRY
卷 298, 期 2, 页码 141-150出版社
ACADEMIC PRESS INC
DOI: 10.1006/abio.2001.5342
关键词
ceramides; diglycerides; DG kinase
资金
- NHLBI NIH HHS [HL-43707] Funding Source: Medline
- NIGMS NIH HHS [R01-GM43825] Funding Source: Medline
This review will discuss the utilization of the diglyceride (DG) kinase assay as an analytical method that allows the simultaneous quantitation of DG and ceramide from cell and tissue samples. This enzymatic approach is sensitive, quantitative, and linear over a broad range (20 pmol to 20-25 nmol) for both classes of lipids. It is also practical in that it can be applied to crude lipid extracts and used to process many samples (up to 100) concurrently. However, it has become apparent that this assay has not been conducted optimally, primarily as a result of lack of adherence to its basic principles. The principles illustrated here are also useful to all enzymatic quantitative methods. (C) 2001 Academic Press.
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