期刊
ANALYTICAL CHEMISTRY
卷 73, 期 22, 页码 5378-5386出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0102157
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资金
- NIEHS NIH HHS [P30-ESO9106] Funding Source: Medline
This paper describes the optimization of sample preparation for MALDI 193-nm photofragment ion time-of-flight mass spectrometry to sequence small to medium-sized peptides from peptide mixtures. We show that matrix additives, such as fructose and phenylbutyric acid have a dramatic effect on the abundance of fragment ions observed in the post-source decay spectra. A dried-droplet MALDI matrix consisting of 1:1 alpha -cyano-4-hydroxycinnamic acid/fructose proves to be an excellent matrix for photodissociation because [M + H](+) ions are formed with low internal energies, and the photofragment ion spectrum contains high abundances of sequence-informative ions. The addition of fructose appears to improve overall sample homogeneity and durability, as compared to conventional alpha -cyano-4-hydroxycinnamic acid dried-droplet preparations. MALDI-TOF photodissociation is then used to selectively sequence the peptides bradykinin (RPPGFSPFR), des-Arg(9) bradykinin (RPPGFSPF), and substance P-amide (PPKPQQFFGLM-NH2) from a mixture of five peptides.
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