期刊
ANALYTICAL BIOCHEMISTRY
卷 298, 期 2, 页码 293-298出版社
ACADEMIC PRESS INC
DOI: 10.1006/abio.2001.5379
关键词
bile acids; evaporative light scattering detector; high performance liquid chromatography
We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids. (C) 2001 Academic Press.
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