期刊
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
卷 176, 期 1-2, 页码 91-94出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.autneu.2013.02.014
关键词
Acetylcholine; Cardiac vagal nerve; Mice; Microdialysis
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [23592691, 24790791]
- Grants-in-Aid for Scientific Research [23592691, 24790791, 23650213, 11J10987] Funding Source: KAKEN
We applied a microdialysis technique to the left ventricular myocardium of anesthetized mice and tried to monitor acetylcholine (ACh) release from cardiac vagal nerves. Transection of bilateral cervical vagal nerves decreased dialysate ACh concentration. Electrical stimulation of the left cervical vagal nerve increased dialysate ACh concentration in proportion to the frequency of stimulation. Intravenous administration of hexamethonium, prevented the increase in dialysate ACh concentration during vagal nerve stimulation, indicating that ACh in the dialysate primarily reflects ACh released from post-ganglionic cardiac vagal nerves. Microdialysis permits monitoring of ACh release from post-ganglionic cardiac vagal nerves that are most likely to be innervating the left ventricle in mice. (C) 2013 Elsevier B.V. All rights reserved.
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