期刊
JOURNAL OF ORGANIC CHEMISTRY
卷 66, 期 23, 页码 7770-7775出版社
AMER CHEMICAL SOC
DOI: 10.1021/jo015890v
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This paper describes the recombinant expression of the ispC gene of Escherichia coli specifying 2C-methyl-D-erythritol 4-phosphate synthase in a modified form that can be purified efficiently by metal-chelating chromatography. The enzyme was used for the preparation of isotope-labeled 2C-methyl-D-erythritol 4-phosphate employing isotope-labeled glucose and pyruvate as starting materials. The simple one-pot methods described afford numerous isotopomers of 2C-methyl-D-erythritol 4-phosphate carrying H-3, C-13, or C-14 from commercially available precursors. The overall yield based on the respective isotope-labeled starting material is approximately 50%.
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