期刊
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
卷 150, 期 1-2, 页码 94-99出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.autneu.2009.05.253
关键词
Urinary bladder; Urothelium; ATP; Extracellular Ca2+; Capsaicin; Acetylcholine; Prostaglandin E-2 receptor EP1
Accumulating evidence shows that the epithelial cells in urinary bladder (urothelium) serve as a sensory organ in micturition and/or in nociception pathway by releasing ATP in response to mechanical and/or chemical stimuli. Here, we compared the effects of capsaicin, acetylcholine, and prostaglandin E-2 receptor EP1 agonist (ONO-DI-004) on the urothelial ATP release in primary cultured mouse urothelial cells in low Ca2+ medium. All of these chemicals induced a gradual ATP release from urothelium, implying that the downstream Ca2+ release from endoplasmic reticulum could trigger the ATP release. Consistent with this suggestion, blockade of inositol 1,4,5-triphosphate receptor reduced the distention-induced ATP release from urothelial tissues. The distention-induced ATP release was not affected by tetrodotoxin. However, an increase in extracellular Ca2+ diminished both chemical- and distention-induced ATP release from urothelium. Thus raising the extracellular Ca2+ concentration was found to inhibit stimulation-evoked ATP urothelial release. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据