期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 12, 期 6, 页码 559-563出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(01)00263-4
关键词
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A cascade of hydantoinase, N-carbamoylase and hydantoinracemase can be used for the production of natural and unnatural chiral D- and L-amino acids from chemically synthesized hydantoin derivatives. Potentially, 100% conversion and 100% optically pure amino acids can be obtained at the same time if racemic substrates are used. Recent research activities concentrate on newly isolated or improved enzymes and include directed evolution techniques, structure elucidation, studies of fusion proteins and the use of specially designed whole cell biocatalysts.
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