期刊
JOURNAL OF VIROLOGY
卷 75, 期 24, 页码 12431-12438出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.75.24.12431-12438.2001
关键词
-
类别
资金
- NIAID NIH HHS [R01 AI043000, AI43000] Funding Source: Medline
To facilitate detection of glycoprotein K (gK) specified by herpes simplex virus, a 12-amino-acid epitope tag was inserted within gK domain III. Recombinant virus gKprotC-DIII, expressing the tagged gK, was isolated. This virus formed wild-type plaques and replicated as efficiently as the wild-type KOS virus in Vero cells. Anti-protein C MAb detected high-mannose and Golgi complex-dependent glycosylated gK within cells as well as on purified virions. The gK-null virus Delta gK (gK(-/-)) entered Vero cells substantially more slowly than the wild-type KOS (gK(+/+)), while Delta gK virus grown in complementing VK302 cells (gK(-/+)) entered with entry kinetics similar to those of the KOS virus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据