期刊
JOURNAL OF CHEMICAL ECOLOGY
卷 27, 期 12, 页码 2545-2558出版社
KLUWER ACADEMIC/PLENUM PUBL
DOI: 10.1023/A:1013687701120
关键词
nectar; amino acid; correlation; HPLC; flowering plant; composition; variation
Thirty species of flowering plants were analyzed for floral nectar amino acid composition. High-performance liquid chromatography (HPLC) was used in conjunction with AccQtag derivatization to produce accurate and precise data. For any one species, the total concentration of amino acids varies greatly (average coefficient of variation 0.65), but composition is much less variable (average correlation among samples from a single species 0.85). Absolute concentration of individual amino acids is much more variable than the relative abundance (coefficients of variation 0.98 and 0.77, respectively; N = 544, t = 16.98. P < 0.001), When amino acids that occur in only small relative abundance (<1%) are removed from the analysis, the difference is even more marked (0.78 and 0.51, respectively; N = 344, t = 15.13, P < 0.001). The results highlight the need for large sample sizes when making observations concerning the absolute amounts of amino acids in nectar and for sensitive analyses of the composition, as even small changes may be biologically significant.
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