期刊
MOLECULAR PLANT-MICROBE INTERACTIONS
卷 14, 期 12, 页码 1453-1457出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI.2001.14.12.1453
关键词
bacterial infection; GCC box; general resistance; transgenic plants
The tomato Pti5 gene encodes a pathogen-inducible ethylene response element-binding protein-like transcription factor that interacts with the disease resistance gene product Pto. Overexpression of Pti5 or Pti5-VP16, a translational fusion with a constitutive transcriptional activation domain, in tomato enhanced resistance to Pseudomonas syringae pv. tomato. Constitutive expression of Pti5 or Pti5-VP16 did not affect the basal level of pathogenesis-related gene expression, but it accelerated pathogen-induced expression of GluB and Catalase. The results demonstrate a positive role of Pti5 in defense gene regulation and disease resistance and suggest that a pathogen-activated posttranscriptional regulatory step is necessary for the pathogen induction of the defense gene expression.
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