期刊
BIORESOURCE TECHNOLOGY
卷 80, 期 3, 页码 187-193出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0960-8524(01)00090-6
关键词
chitosan beads; shrimp shell wastes; cross-linking; adsorption; dye; Cu(II); immobilization; acid phosphatase
The equilibrium and kinetics of adsorption of reactive dye RR222 and Cu2+, and the activity of immobilization of acid phosphatase, on highly swollen chitosan beads were examined at 30 degreesC. The chitosan was prepared from shrimp shell wastes and was cross-linked with different dosages of glutaraldehyde or glyoxal (100-80,000 mg/l). It was shown that the amounts of solute adsorption and the immobilization capacity of acid phosphatase on cross-linked chitosan beads were substantially affected by their degree of cross-linking. The cross-linking rate of chitosan with glutaraldehyde could be described by a pseudo-second-order equation and the cross-linking equilibrium by the Freundlich equation. This provided an experimental method to control the degree of cross-linking of chitosan beads. Finally, the activity and lifetime of the immobilized enzyme were measured to evaluate the application potential. (C) 2001 Elsevier Science Ltd. All rights reserved.
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