期刊
WATER RESEARCH
卷 35, 期 18, 页码 4417-4425出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0043-1354(01)00178-6
关键词
16S rRNA; DGGE; mesophilic; PCR; similarity analysis; thermophilic
The effect of temperature was studied on the efficiency of soluble COD removal and bacterial community development during the aerobic biological treatment of a pharmaceutical wastewater. Using wastewater and bacterial inoculum obtained from the full-scale facility treating this wastewater, batch laboratory cultures were operated at 5 degreesC intervals from 30 degreesC to 70 degreesC. Following four culture transfers to allow for bacterial acclimation, residual soluble COD levels were measured and bacterial community fingerprints were obtained by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments. Soluble COD removal efficiency declined as temperature increased from 30 degreesC (62%) to 60 degreesC (38%). Biological treatment of this wastewater failed to occur at temperatures higher than 60 degreesC. Gradual shifts in bacterial community structure were detected as temperature increased, including a concomitant reduction in the number of different bacterial populations. The impact of temperature on a two-stage biological treatment process was also compared. Better soluble COD removal was achieved when both reactors were operated at 30 degreesC compared to a system where the two stages were consecutively operated at 55 degreesC and 30 degreesC. These results indicate that operation of aerobic biological wastewater treatment reactors at elevated temperatures can have adverse effects on process performance. (C) 2001 Elsevier Science Ltd. All rights reserved.
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