期刊
COMPARATIVE AND FUNCTIONAL GENOMICS
卷 2, 期 6, 页码 359-364出版社
HINDAWI LTD
DOI: 10.1002/cfg.113
关键词
surface plasmon resonance; mass spectrometry; protein-protein interaction; endopolygalacturonase; Fusarium moniliforme; PGIP
A combination of surface plasmon resonance (SPR) and matrix-assisted laser-desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) was used to study the interaction between endopolygalacturonase (PG) from Fusarium moniliforme and a polygalacturonase-inhibiting protein (PGIP) from Phaseolus vulgaris. PG hydrolyses the homogalacturonan of the plant cell wall and is considered an important pathogenicity factor of many fungi. PGIP is a specific inhibitor of fungal PGs and is thought to be involved in plant defence against phytopathogenic fungi. SPR was used either to study the effect of the PG glycosylation on the formation of the complex with PGIP, and as a sensitive affinity capture of an interacting peptide from a mixture of PG fragments obtained by limited proteolysis. Mass spectrometry allowed to characterise the interacting peptide eluted from the sensor surface. Copyright (C) 2001 John Wiley Sons, Ltd.
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