期刊
MOLECULAR ECOLOGY NOTES
卷 1, 期 4, 页码 297-299出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-8278.2001.00116.x
关键词
enrichment; forest tree; Maloideae; Rosaceae; Sorbus torminalis
This study reports the cloning and characterization of nine microsatellite primer pairs in a scattered woody species (Sorbus torminalis), and shows their potential for further use in 36 species of the Maloideae, a Rosaceae subfamily containing important fruit crop and ornamental species. These primers were designed from a microsatellite library constructed from genomic DNA of S. torminalis and enriched for CA and GA repeats. Genotyping 48 S. torminalis of a natural population with the six best markers yielded a mean of 10.7 alleles per locus, and an expectation of exclusion probability for paternity analysis greater than 0.993.
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