期刊
TOXICOLOGY IN VITRO
卷 15, 期 6, 页码 649-654出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0887-2333(01)00081-9
关键词
mercuric chloride; genotoxicity; ascorbic acid; human blood cultures
类别
Efforts are made to find therapeutic agents capable of minimizing genotoxicity of various natural and man-made compounds. The genotoxicity induced by mercury compounds remains controversial. Therefore we have investigated the genotoxic effect of mercuric chloride (NIC; HgCl2) at three concentrations (1.052, 5.262 and 10.524 muM) and role of L-ascorbic acid (vitamin C) at a concentration of 9.734 muM on MC-treated short-term human leucocyte cultures. We assessed the proliferative rate index (PRI), sister chromatid exchange (SCE) and chromosomal aberrations (CAS) in control and MC-treated cultures with and without vitamin C supplementation. The results showed that MC has no effect on cell-cycle kinetics, but the frequency of SCE/cell was significantly higher in a dose-dependent manner than control values. HgCl2 also significantly induced C-anaphases (abnormal mitosis) in blood cultures. These effects were prevented by the addition of vitamin C to MC-treated cultures. The data indicate the mutagenic activity of MC and the protective role of vitamin C on mercury-induced genotoxicity in human blood cultures is probably due to its strong antioxidant and nucleophilic nature. (C) 2001 Elsevier Science Ltd. All rights reserved.
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