期刊
ARCHIVES OF MICROBIOLOGY
卷 176, 期 6, 页码 435-442出版社
SPRINGER
DOI: 10.1007/s002030100343
关键词
ether lipids; sulfate-reducing bacteria; phospholipids; HPLC-ESI-MS
类别
In this study, cellular lipid compositions of two mesophilic sulfate-reducing bacteria were analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS). In Desulfosarcina variabilis and Desulforhabdus amnigenus, alkylether-containing phospholipids were detected which had previously only been found in significant amounts in deeply branching hyperthermophilic bacteria and archaea. Combining information from HPLC-MS analysis and chemical degradation experiments, ether lipids were identified as 1-alkyl-2-acyl-phosphatidyl ethanolamines, glycerols and cholines. In Desulforhabdus amnigenus, n-penta-, n-hexa- and n-heptadecyl ethers were present (in order of decreasing abundance); whereas Desulfosarcina variabilis solely contained n-hexadecyl ether side chains.
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