期刊
MOLECULAR CELL
卷 8, 期 6, 页码 1383-1390出版社
CELL PRESS
DOI: 10.1016/S1097-2765(01)00423-3
关键词
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资金
- NIAID NIH HHS [AI 20047, AI 35714] Funding Source: Medline
RAG1 and RAG2 (RAGs) initiate V(D)J recombination by introducing breaks between two coding segments and flanking recombination signals (RSs). Nonhomologous end-joining (NHEJ) proteins then join the coding segments and join the RSs. In wild-type cells, both full-length and truncated (core) RAGs lead to accumulation of hybrid V(D)J joins, in which an RS is appended to a different coding sequence. We now show that while hybrid joins do not accumulate in NHEJ-deficient cells that express full-length RAGs, they do accumulate in NHEJ-deficient cells that express the core RAGS; like those catalyzed by core RAGs in vitro, however, they are sealed on just one DNA strand. These results suggest a potential role for the non-core regions in repressing potentially harmful transposition events.
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