期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 65, 期 6, 页码 936-942出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2001.65.936
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资金
- NIAID NIH HHS [AI 21242] Funding Source: Medline
Phospholipase A, activity by typhus group rickettsiae causes hemolysis in vitro. Rickettsial phospholipase A(2) has been proposed to mediate entry into the host cell, escape from the phagosome, and cause injury to host cells by both typhus and spotted fever group rickettsiae. In a rickettsial contact-associated cytotoxicity model, the interaction of Rickettsia prowazekii or R. conorii with Vero cells caused temperature-dependent release of Cr-51 from the cells. Treatment of rickettsiae, but not the cells, with a phospholipase A, inhibitor (bromophenacyl bromide) or with antibody to king cobra venom inhibited cell injury. Rickettsial treatment with bromophenacyl bromide inhibited the release of free fatty acids from the host cell. Neither the inhibitor nor antivenom impaired rickettsial active transport of L-lysine. Thus, host cell injury was mediated by a rickettsial phospholipase A(2)-dependent mechanism.
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