期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 281, 期 6, 页码 H2295-H2303出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.2001.281.6.H2295
关键词
protein kinase A; ryanodine receptor; nitrosylation; excitation-contraction coupling
资金
- NHLBI NIH HHS [HL-64098, HL-10122, HL-34724] Funding Source: Medline
Nitric oxide (NO) can have a positive or negative effect on cardiac contractility and the ryanodine receptor (RyR). This dual effect has been explained as being dependent on the concentration of NO. We find that cellular RyR response to NO is also dependent on the degree of beta -adrenergic stimulation, and thus the state of protein kinase A activation. Ca2+ spark frequency (CaSpF) in rat ventricular myocytes was used as an index of resting RyR activity. CaSpF response to beta -adrenergic stimulation was used as an index of protein kinase A activation. High concentration of isoproterenol, a beta -adrenergic agonist, caused a large increase in CaSpF; addition of NO (spermine NONO-ate, 300 muM) then caused a decrease in CaSpF. Low concentration of isoproterenol produced only a slight increase in CaSpF, but the same NO concentration now caused a large increase in CaSpF. A dual effect was also observed in twitch. Thus the net direction of the effects of NO on RyR activity and Ca2+ transients (directly or by alteration of sarcoplasmic reticulum Ca2+ load) can be reversed, depending on the ambient level of beta -adrenergic activation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据