期刊
BIOCHEMICAL JOURNAL
卷 360, 期 -, 页码 431-439出版社
PORTLAND PRESS LTD
DOI: 10.1042/0264-6021:3600431
关键词
beta-cell granules; perfused pancreas; proIGF-II E-peptide; protein mass spectrometry; secretory granules
Pancreatic islet beta -cells secrete the hormones insulin, amylin and pancreastatin. To search for further beta -cell hormones, we purified peptides from secretory granules isolated from cultured murine beta TC6-F7 beta -cells. We identified a 34-amino-acid peptide (3948 Da), corresponding to Asp(69)-Leul(102) of the proinsulin-like growth factor II E-peptide, which we have termed 'preptin'. Preptin, is present in islet beta -cells and undergoes glucose-mediated co-secretion with insulin. Synthetic preptin increases insulin secretion from glucose-stimulated beta TC6-F7 cells in a concentration-dependent and saturable manner. Preptin infusion into the isolated, perfused rat pancreas increases the second phase of glucose-mediated insulin secretion by 30%, while antipreptin immunoglobulin infusion decreases the first and second phases of insulin secretion by 29 and 26% respectively. These findings suggest that preptin is a physiological amplifier of glucose-mediated insulin secretion.
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