期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 98, 期 25, 页码 14398-14403出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.251532698
关键词
-
The photophysics and photochemistry taking place in the DsRed protein, a recently cloned red fluorescent protein from a coral of the Discosoma genus, are investigated here by means of ensemble and single-molecule time-resolved detection and spectroscopic measurements. Ensemble time-resolved data reveal that 25% of the immature green chromophores are present in tetramers containing only this immature form. They are responsible for the weak fluorescence emitted at 500 nm. The remaining 75% of the immature green chromophores are involved in a fluorescence resonance energy transfer process to the red species. The combination of time-resolved detection with spectroscopy at the single-molecule level reveals, on 543-nm excitation of individual DsRed tetramers, the existence of a photoconversion of the red chromophore emitting at 583 nm and decaying with a 3.2-ns time constant into a super red one emitting at 595 nm and for which the decay time constant ranges between 2.7 and 1.5 ns. The phenomenon is further corroborated at the ensemble level by the observation of the creation of a super red form and a blue absorbing species on irradiation with 532-nm pulsed light at high excitation power. Furthermore, single-molecule experiments suggest that a similar photoconversion process might occur in the immature green species on 488-nm excitation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据