Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry

A liquid chromatographic procedure with electrospray ionization tandem mass spectrometric detection has been developed and validated for LSD and iso-LSD determination. A one-step Equid-liquid extraction on I ml blood or urine was used. The lower limit for quantitative determination was 0.02 mug/l for LSD and iso-LSD. The analytical procedure has been applied in two positive cases (case 1: LSD=0.31 mug/l, iso-LSD=0.27 mug/l in plasma and LSD=1.30 mug/l, iso-LSD=0.82 mug/l in urine; case 2: LSD=0.24 mug/l, iso-LSD=0.6 mug/l in urine). LSD metabolism was investigated using MS-MS neutral loss monitoring for the screening of potential metabolites. The main metabolite was 2-oxo-3-hydroxy-LSD (O-H-LSD) present in urine at the concentrations of 2.5 mug/l and 6.6 mug/l, respectively, for case 1 and 2, and was not present in plasma. Nor-LSD was also found in urine at 0.15 and 0.01 mug/l levels. Nor-iso-LSD, lysergic acid ethylamide (LAE), trioxylated-LSD, lysergic acid ethyl-2-hydroxyethylamide (LEO) and 13 and 14-hydroxy-LSD and their glucuronide conjugates were detected in urine using specific MS-MS transitions. (C) 2001 Elsevier Science B.V. All rights reserved.