期刊
AUTOIMMUNITY
卷 46, 期 3, 页码 222-230出版社
INFORMA HEALTHCARE
DOI: 10.3109/08916934.2012.751981
关键词
Tregs; Graves' disease; Hashimoto's thyroiditis; children
类别
The essence of autoimmune thyroid disease (AITD) is loss of tolerance of own tissues caused by malfunction of T lymphocytes, which affects the production of antibodies reacting with particular cell structures and tissues. Foxp3(+) regulatory T cells (Tregs) take part in the regulation of immune response and play a leading role in developing immune tolerance through active suppression. The aim of the study was to estimate the expression of CD4 + CD25(high), CD4 + CD25 + CD127(low) FoxP3(+) and CD4 + FoxP3 T cells in patients with Graves' disease (GD) (n = 24, median age 15.5 years), in patients with Hashimoto's thyroiditis (HT) (n = 30, median age 15 years) in comparison with sex-and age-matched healthy control subjects (n = 30, median age 15 years). Polychromatic flow cytometry using a FACSCalibur (BD Biosciences) cytometer was applied to delineate T regulatory cell populations. In untreated patients with Graves' disease and HT we observed a significant decrease in CD4 + FoxP3 (p < 0.001, p < 0.01) and CD4 + CD25(high) (p < 0.016, p < 0.048) T lymphocytes as compared to the healthy control subjects. After 6-12 months of L-thyroxine therapy in HT cases these phenotypes of Tregs were normalized, yet no such changes were observed during GD therapy. The analysis of CD4 + CD25 + CD127(low) FoxP3(+)T cells in the peripheral blood revealed comparable percentages of these cells in patients with thyroid autoimmune diseases to the controls. We conclude that the reduction number of Tregs with CD4 + CD25(high) and CD4 + FoxP3 phenotype suggests their role in initiation and development of autoimmune process in thyroid disorders.
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