期刊
CELLULAR IMMUNOLOGY
卷 214, 期 2, 页码 110-122出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/cimm.2001.1895
关键词
dendritic cell; CD8(+) T cell; transgenic mouse; green fluorescent protein; UbC promoter; adoptive transfer; histology; immunofluorescence microscopy; flow cytometry
资金
- NIAID NIH HHS [AI22295, AI17134, AI18785] Funding Source: Medline
In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with high levels of GFP expression observed in hematopoetic cells. UBI-GFP/BL6 mice are unique in that B cells, T cells, and dendritic cells have distinct levels of GFP fluorescence. In cell transfer experiments, leukocytes from UBI-GFP/BL6 mice are readily identified by FACS or fluorescence microscopy. We demonstrate that transplanted UBI-GFP/BL6 dendritic cells are easily identified in secondary lymphoid tissues. Direct interactions between individual dendritic cells and multiple naive CD8(+) T cells are observed in lymph nodes within 12 h of cell transfer and require loading of the dendritic cells with the appropriate peptide antigen. Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T Cells. (C) 2001 Elsevier Science (USA).
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