期刊
EMBO JOURNAL
卷 20, 期 24, 页码 7259-7270出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/20.24.7259
关键词
reverse transcriptase; ribozyme; RNA catalysis; RNA folding; RNA-protein interaction
资金
- NIGMS NIH HHS [GM37949, R01 GM037949] Funding Source: Medline
Mobile group II introns encode reverse transcriptases that also function as intron-specific splicing factors (maturases). We showed previously that the reverse transcriptase/maturase encoded by the Lactococcus lactis LI.LtrB intron has a high affinity binding site at the beginning of its own coding region in an idiosyncratic structure, DIVa. Here, we identify potential secondary binding sites in conserved regions of the catalytic core and show via chemical modification experiments that binding of the maturase induces the formation of key tertiary interactions required for RNA splicing. The interaction with conserved as well as idiosyncratic regions explains how maturases in some organisms could evolve into general group II intron splicing factors, potentially mirroring a key step in the evolution of spliceosomal introns.
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