期刊
NEUROSCIENCE LETTERS
卷 316, 期 2, 页码 67-70出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(01)02384-9
关键词
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine -; a dopaminergic neurotoxin; bone marrow stromal cells; transplantation; Rotarod test; mouse
资金
- NINDS NIH HHS [P01 NS23393] Funding Source: Medline
Adult C57BL/6 mice were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Intrastriatal transplantation of bone marrow stromal cells (MSCs) was performed 1 week after MPTP administration. MSCs were harvested from donor adult mice, and then cultured and prelabeled with bromodeoxyuridine (BrdU). MPTP-Parkinson's disease (PD) mice treated with intrastriatal injection of phosphate-buffered saline (PBS), and normal non-MPTP mice were used as controls. MPTP-PD mice with MSC intrastriatal transplantation exhibit significant improvement on the rotarod test (P < 0.05) at day 35 compared with PBS controls. Immunohistochemistry shows that BrdU reactive cells survive in the transplanted areas in the MPTP-PD striatum at least 4 weeks after administration. Scattered BrdU reactive cells express tyrosine hydroxylase (TH) immunoreactivity. Our findings suggest that MSCs injected intrastriatally survive, express dopaminergic protein TH immunoreactivity, and promote functional recovery. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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