期刊
FEMS MICROBIOLOGY LETTERS
卷 206, 期 2, 页码 197-200出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1097(01)00525-0
关键词
DNA microarray; nitrogen starvation; competence; transformation procedure; Bacillus subtilis
类别
DNA microarrays were used to analyze the changes in gene expression in Bacillus subtilis strain 168 when nitrogen limiting (glutamate) and nitrogen excess (ammonium plus glutamate) growth conditions were compared. Among more than 100 genes that were significantly induced during nitrogen starvation we detected the comG, comF, comE, nin-nucA and comK transcription units together with recA. DNA was added to B. subtilis grown in minimal medium with glutamate as the sole nitrogen source and it was demonstrated that the cells were competent. Based on these observations we propose a simplification of previously designed one-step transformation procedures for B. subtilis strain 168. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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