期刊
EMBO JOURNAL
卷 21, 期 1-2, 页码 22-30出版社
WILEY-BLACKWELL
DOI: 10.1093/emboj/21.1.22
关键词
hemolysis; human red blood cells; malaria; new permeability pathway; patch-clamp
Intraerythrocytic survival of the malaria parasite Plasmodium falciparum requires that host cells supply nutrients and dispose of waste products. This solute transport is accomplished by infection-induced new permeability pathways (NPP) in the erythrocyte membrane. Here, whole-cell patch-clamp and hemolysis experiments were performed to define properties of the NPP. Parasitized but not control erythrocytes constitutively expressed two types of anion conductances, differing in voltage dependence and sensitivity to inhibitors. In addition, infected but not control cells hemolyzed in isosmotic sorbitol solution. Both conductances and hemolysis of infected cells were inhibited by, reducing agents. Conversely, oxidation induced identical conductances and hemolysis in non-infected erythrocytes. In conclusion, P. falciparum activates endogenous erythrocyte channels by applying oxidative stress to the host cell membrane.
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