期刊
FEBS LETTERS
卷 511, 期 1-3, 页码 79-84出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)03300-2
关键词
hepatitis C virus; internal ribosome entry site; translation; domain II; apical loop; mutational analysis
The hepatitis C virus internal ribosome entry site (IRES) binds directly to the 40S ribosomal subunit via domains III/IV while domain II induces conformational changes on the ribosome which have been implicated in the decoding process. Here, we performed an extensive mutational study within the apical portion of domain II in order to address the functional role of this region on translation. Our results showed that the conservation of most nucleotides in this region was only partially related to the IRES function. Notwithstanding, however, selected single point mutations within the apical loop had a deleterious effect on IRES activity. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据