期刊
YEAST
卷 19, 期 3, 页码 215-224出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/yea.810
关键词
Saccharomyces cerevisiae; nicotinamidase; PNC1 gene; stress response element
Nicotinamidase (NAMase) from the budding yeast, Saccharomyces cerevisiae, was purified by Ni2+ affinity chromatography and gel filtration. N-terminal microsequencing revealed sequence identity with a hypothetical polypeptide encoded by the yeast YGL037C open reading frame sharing 30% sequence identity with Escherichia coli pyrazinamidase/nicotinamidase. A yeast strain in which the NAMase gene, hereafter named PNC1, was deleted shows a decreased intracellular NAD(+) concentration, consistent with the loss of NAMase activity in the null mutant. In wild-type strains, NAMase activity is stimulated during the stationary phase of growth, by various hyperosmotic shocks or by ethanol treatment. Using a P-PNC1::lacZ gene fusion, we have shown that this stimulation of NAMase activity results from increased levels of the protein and requires stress response elements in the 5' non-coding region of PNC1. These results suggest that NAMase helps yeast cells to adapt to various stress conditions and nutrient depletion, most likely via the activation of NAD-dependent biological processes. Copyright (C) 2002 John Wiley Sons, Ltd.
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