Bovine serum albumin-bovine hemoglobin conjugate as a candidate blood substitute

A bovine serum albumin-bovine hemoglobin conjugate was prepared using 3-maleimidobenzoic acid N-hydroxysuccinimide ester as cross-linker. The conjugate was purified using DEAE Sepharose. It had an M-r of 127 kDa. Its P(5)0 (half-saturated O-2 pressure) value and Hill coefficient were 27 mm Hg and 2, respectively.