期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 32, 期 3, 页码 228-232出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0891-5849(01)00795-X
关键词
free radicals; EPR; nitrone; spin trapping
A previous report that the spin trap 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) allows DEPMPO radical cation formation to be detected via the production of a carbon-centred radical adduct (assigned as the cis-hydroxyethyl species, formed by an intramolecular process) is shown to be incorrect. Rather, this and other paramagnetic species arise from the facile oxidation of trace hydroxylamine impurities present in commercial DEPMPO samples. As a result, techniques for the detection and elimination of such hydroxylamine impurities from DEPMPO solutions were developed and are described these should prove to be of general use in EPR spin trapping experiments. (C) 2002 Elsevier Science Inc.
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