期刊
WATER RESEARCH
卷 36, 期 3, 页码 638-646出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0043-1354(01)00277-9
关键词
16S rRNA; community analysis; DGGE; PCR; treatment; wastewater
The stabilities of the bacterial community structures supported by seven full-scale biological reactors treating pharmaceutical wastewater were investigated by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR) amplified 16S rRNA gene fragments. Effluent quality from this treatment process was consistently high with respect to BOD5 (<30 mg l(-1)), soluble COD (< 500 mg l(-1)). and total ammonia (<5 mg l(-1) as N) concentrations. Long-term community structure stability was studied by comparing the similarity of PCR-DGGE fingerprints from samples collected 87 days apart between which the influent wastewater characteristics were relatively stable. The Dice index (C-s) of similarity was moderately high for the first four reactors (C-s = 0.61-0.77) and very high for the last three reactors (C-s = 0.89-0.91). Short-term community structure stability was studied by comparing PCR-DGGE fingerprints from samples collected 15 days apart between which the influent wastewater characteristics changed significantly, while the effluent quality remained consistently high, The bacterial community composition of each of the seven bioreactors showed a moderate community shift (C-s = 0.70-0.76). Short-term variability in influent wastewater composition, therefore, affected a greater community shift than did long-term operation treating a wastewater of relatively consistent composition. These results indicate that functionally stable wastewater treatment bioreactors have stable microbial community structures under normal operating conditions but are able to adapt in response to perturbations to sustain high effluent quality. (C) 2002 Elsevier Science Ltd. All rights reserved.
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