期刊
GASTROENTEROLOGY
卷 122, 期 2, 页码 415-427出版社
W B SAUNDERS CO
DOI: 10.1053/gast.2002.30982
关键词
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资金
- FIC NIH HHS [TW01452] Funding Source: Medline
- NCRR NIH HHS [RR04224] Funding Source: Medline
- NIDDK NIH HHS [DK45710, DK57751] Funding Source: Medline
Background & Aims: The inositol 1,4,5-trisphosphate (InsP(3)) receptor (InsP(3)R) and the ryanodine receptor (RyR) are the principal Ca2+-release channels in cells and are believed to serve distinct roles in cytosolic Ca2+ (Ca-i(2+)) signaling. This study investigated whether these receptors instead can release Ca2+ in a coordinated fashion. Methods: Apical and basolateral Ca-i(2+) signals were monitored in rat pancreatic acinar cells by time-lapse confocal microscopy. Caged forms of second messengers were microinjected into individual cells and then photoreleased in a controlled fashion by either UV or 2-photon flash photolysis. Results: InsP(3) increased Ca-i(2+) primarily in the apical region of pancreatic acinar cells, whereas the RyR agonist cyclic adenosine diphosphate ribose (cADPR) increased Ca-i(2+) primarily in the basolateral region. Apical-to-basal Ca-i(2+) waves were induced by acetylcholine and initiation of these waves was blocked by the InsP(3)R inhibitor heparin, whereas propagation into the basolateral region was inhibited by the cADPR inhibitor 8-amino-cADPR. To examine integration of apical and basolateral Ca-i(2+) signals, Ca2+ was selectively released either apically or basolaterally using 2-photon flash photolysis. Ca-i(2+) increases were transient and localized in unstimulated cells. More complex Ca-i(2+) Signaling patterns, including polarized Ca-i(2+) waves, were observed when Ca2+ was photoreleased in cells stimulated with subthreshold concentrations of acetylcholine. Conclusions: Polarized Ca-i(2+) waves are induced in acinar cells by serial activation of apical InsP(3)Rs and then basolateral RyRs, and subcellular release of Ca2+ coordinates the actions of these 2 types of Ca2+ channels. This subcellular integration of Ca2+-release channels shows a new level of complexity in the formation of Ca-i(2+) waves.
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