期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1558, 期 2, 页码 119-132出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0005-2736(01)00418-7
关键词
red blood cell; lysis; hypotonic medium; capacitance/conductance
Red blood cell (RBC) swelling and membrane hole formation in hypotonic external media were studied by measuring the time-dependent capacitance, C, and the conductance, G, in the beginning of the beta-dispersion range. At high and moderate osmolarities of the external solution the capacitance reaches a steady-state whereas at low osmolarities it reveals a biphasic kinetics. Examination of RBC suspensions exposed to different concentrations of HgCl2 demonstrates that water transport through mercury-sensitive water channel controls RBC swelling. Unlike the capacitance, an increase in the conductance to a stationary level is observed after a certain delay. A comparison of G(t) curves recorded for the suspensions of the intact cells and those treated with cytochalasin B or glutaraldehyde demonstrates the significant effect of the membrane viscoelasticity on the pore formation. It is shown that the stretched membrane of completely swollen RBC retains its integrity for a certain time, termed as the membrane lifetime. t(memb). Therefore, the resistivity of RBCs to a certain osmotic shock. may be quantified by the distribution function of RBC(t(memb)). (C) 2002 Elsevier Science B.V. All rights reserved.
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