期刊
TRANSGENIC RESEARCH
卷 11, 期 1, 页码 31-41出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1013944011049
关键词
coat protein gene (CP)-mediated resistance; pathogen-derived resistance; PTGS (post-transcriptional gene silencing); wheat transformation; WSMV
Wheat (Triticum aestivum) plants were stably transformed with the coat protein (CP) gene of wheat streak mosaic virus (WSMV) by the biolistic method. Eleven independently transformed plant lines were obtained and five were analyzed for gene expression and resistance to WSMV. One line showed high resistance to inoculations of two WSMV strains. This line had milder symptoms and lower virus titer than control plants after inoculation. After infection, new growth did not show symptoms. The observed resistance was similar to the 'recovery' type resistance described previously using WSMV NIb transgene and in other systems. This line looked morphologically normal but had an unusually high transgene copy number (approximately 90 copies per 2C homozygous genome). Northern hybridization analysis indicated a high level of degraded CP mRNA expression. However, no coat protein expression was detected.
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