期刊
CLINICAL ORAL IMPLANTS RESEARCH
卷 13, 期 1, 页码 86-93出版社
WILEY-BLACKWELL
DOI: 10.1034/j.1600-0501.2002.130111.x
关键词
implant; titanium; monocytes; TNF-alpha; PGE(2); surface roughness; cell adhesion
Dental implant surfaces are important in determining the tissue/surface interaction. one of the first cells to adhere to the implant surface is the monocyte. T is study examines the effect of surface roughness on monocyte adhesion and cytokine secretion. Monocyte adherence to titanium discs of 4 different degrees of surface roughness and plastic surfaces was assayed, Blood mononuclear cells were incubated for 1.5 h in 16 mm culture wells into which titanium discs had been placed. Non-adherent cells were washed off and the numbers of remaining adherent monocyte determined by DNA quantification. TNF-alpha and PGE(2) secretion in media from overnight cultures of attached monocytes stimulated with lipopolysaccharide (LPS) was quantified using ELISA and RIA, respectively. Monocyte adherence to rough titanium surfaces was greater than to turned titanium surfaces, while the lowest adherence was to the plastic surface. No significant differences in adherence to 250, 75 or 25 mum blasted surfaces could be detected. The number of adherent monocytes increased with time, with maximum adhesion after 2 h of incubation. Incubation of monocytes adherent to titanium surfaces resulted in a decrease of less than 30% in their numbers over 7 days, whereas cells attached to plastic surfaces decreased to non-detectable numbers after 48 h. Porphyromonas gingivalis LPS stimulation upregulated TNF-alpha and PGE(2) secretion into the media. The LPS-induced TNF-alpha and PGE(2) secretion was independent of the titanium surface roughness, however the lowest amounts of TNF-alpha and PGE(2) were secreted from cells attached to plastic surfaces. The results of this study indicate that the number of monocytes attached to blasted titanium surfaces is significantly greater than to machined titanium surfaces. PGE(2) and TNF-alpha secretion is less influenced by titanium surface roughness.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据