期刊
AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH
卷 18, 期 2, 页码 239-244出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1755-0238.2012.00188.x
关键词
nucleic acid extraction; sampling; viticulture
资金
- Grape and Wine Research and Development Corporation
- Department of Primary Industries, Victoria
- South Australian Research and Development Institute
Background and Aims: Australian certification programs that provide high-health planting material depend on accurate virus detection methods. The reliability of enzyme-linked immunosorbent assays (ELISAs) and reverse transcription-polymerase chain reaction (RT-PCR) tests for virus detection was compared in Australian conditions. Methods and Results: Replicate trials were established in a hot climate and a cool climate with grapevines that were uninoculated or inoculated with Grapevine virus A, Grapevine fleck virus, Grapevine leafroll-associated virus (GLRaV)-2 and GLRaV-3. Grapevines were tested monthly for virus during 3 years. RT-PCR detected viruses more frequently than ELISA, and the reliability of both tests increased after 12 months and up to 3 years post-inoculation in both climates. Conclusions: Viruses may not be consistently detected until 12 months after an infection event. RT-PCR is more reliable than ELISA for virus detection during spring and summer. However, detection of viruses was rarely 100% efficient, and retesting of grapevines is recommended to improve the rate of detection. Significance of the Study: Validated diagnostic procedures were developed to improve the reliability of grapevine virus detection in Australia.
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