期刊
BIOTECHNOLOGY LETTERS
卷 24, 期 4, 页码 251-256出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1014076426705
关键词
antimicrobial; expression; peptide; screening
The genetic library of alpha-helical amphipathic peptides, 20 amino acid long, was designed and expressed under the T7 promoter in the E. coli JM109(DE3) and BL21 (DE3). Clones that inhibited the growth of the host cell were screened by the relative size of colonies on the plates. Clones which strongly inhibited growth of Escherichia coli JM109(DE3) were further selected. The method developed in this study is useful for the structure activity relationship study of antimicrobial peptides.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据