期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 30, 期 2, 页码 224-230出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(01)00483-5
关键词
alcohol acetyltransferase; ester hydrolase; headspace-SPME
A method has been developed for measurement in vitro of the simultaneous activities alcohol acetyltransferase (AATase) and ester hydrolase (EHase) in yeast extracts taking into account the possibility of interaction between the two opposite activities or the rapid inactivation of the AATase activity. A mathematical model. including as parameters a first order kinetic constant corresponding to the EHase activity and the inactivation constant of AATase. is proposed for the evaluation of AATase activity. To determine ester concentrations, the Headspace-SPME-GC technique has been used. The method has been successfully applied to three yeast strains belonging to the species Pichia anomala, Pichia heedii and Saccharomyces cerevisiae and the corresponding parameters for AATase and EHase have been calculated. (C) 2002 Elsevier Science Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据