期刊
ANALYTICA CHIMICA ACTA
卷 453, 期 1, 页码 33-41出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(01)01483-0
关键词
ochratoxin A; wine; mass spectrometry fluorescence; solid-phase extraction reversed-phase; immunoaffinity
Different analytical methods for the determination of ochratoxin A (OTA) in. wine have been compared. Sample clean-up was based on solid-phase extraction (SPE) with (i) immunoaffinity or (ii) RP-18 sorbent materials applying different experimental protocols. The detection of OTA was accomplished with high-performance liquid chromatography (HPLC) combined either with electrospray ionisation (ESI) tandem mass spectrometry (MS-MS) or fluorescence detection (FL). Comparative method evaluation was based on the investigation of 118 naturally contaminated red wine samples originating from different European countries. The analytical results are discussed in view of the respective method validation data and the corresponding experimental protocols. In general, analytical data obtained with RP-18 SPE combined with LC-MS-MS detection and immunoaffinity extraction combined with FL offered comparable good results in the sub-ppb concentration level indicating that high selectivity of either the sample clean-up or, alternatively the detection system are equally well-suited to guarantee an accurate OTA analysis in wine. (C) 2002 Elsevier Science B.V. All rights reserved.
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