期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 99, 期 4, 页码 2100-2105出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.032262999
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integration of foreign DNA was observed in the Gram-positive human pathogen Streptococcus pneumoniae(pneumococcus) after transformation with DNA from a recombinant Escherichia coli bacteriophage A carrying a pneumococcal insert. Segments of A DNA replaced chromosomal sequences adjacent to the region homologous with the pneumococcal insert, whence the name insertion-deletion. Here we report that a pneumococcal insert was absolutely required for insertion-deletion formation, but could be as short as 153 bp; that the sizes of foreign DNA insertions (289-2,474 bp) and concomitant chromosomal deletions (45-1,485 bp) were not obviously correlated; that novel joints clustered preferentially within segments of high GC content; and that the crossovers in 29 independent novel joints were located 1 bp from the border or within short (3-10 nt long) stretches of identity (microhomology) between resident and foreign DNA. The data are consistent with a model in which the insert serving as a homologous recombination anchor favors interaction and subsequent illegitimate recombination events at microhomologies between foreign and resident sequences. The potential of homology-directed illegitimate recombination for genome evolution was illustrated by the trapping of functional heterologous genes.
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