期刊
VIRUS RESEARCH
卷 83, 期 1-2, 页码 1-12出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1702(01)00379-3
关键词
measles virus; matrix protein; epithelial cells; intracellular transport
类别
As we have shown earlier, the measles virus (MV) glycoproteins H and F are expressed on both, the apical and the basolateral membrane of polarized Madin-Darby canine kidney cells. In contrast to the glycoproteins, we found the viral matrix protein (M) to accumulate selectively at the apical plasma membrane of MV-infected cells. M did not colocalize with the glycoproteins at basolateral membranes of polarized cells indicating an independent surface transport mechanism. Analysis of infected cells treated with monensin supported this view. When H and F were retained in the medial Golgi by monensin treatment, M did not accumulate in this cellular compartment. To elucidate the subcellular transport mechanism of the cytosolic M protein, M was expressed in the absence of other viral proteins. Flotation analysis demonstrated that most of the M protein coflotated in infected or in M-transfected cells with cellular membranes, Thus, the M protein possesses the intrinsic ability to bind to lipid membranes. Unexpectedly, plasmid-encoded M protein was rarely found to accumulate at surface membranes. Although cotransport with the viral glycoproteins was not needed, M transport to the plasma membrane required a component only provided in MV-infected cells. (C) 2002 Elsevier Science B.V. All rights reserved.
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