期刊
SCIENTIA HORTICULTURAE
卷 93, 期 1, 页码 19-28出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-4238(01)00309-0
关键词
Vitis; interspecific cross; RAPD marker; inheritance
类别
Inheritance of randomly amplified polymorphic DNA (RAPD) markers in an interspecific F, hybrid between Vitis quinquangularis ('83-4-96') and V. vinifera ('Muscat Rose') were studied. A preliminary screen of the F, plants and the parents used as the interspecific cross parent was conducted with 280 RAPD primers; of these, 39 primers were polymorphic and reproducible and produced 234 scorable loci. Genomic DNA from 60 individuals of the F-1 population was then amplified with these primers; 83.4% of the loci segregated as expected in a Mendelian fashion. According to the segregation patterns, RAPD markers were grouped into three types: ( 1) normal Mendelian inheritance with segregation ratio of nearly 1: 1, 3:1 or 1:0 (non-segregation); (2) deviation from Mendelian segregation ratios; (3) abnormal segregation in low frequency, including non-parental markers which were present in F, progenies but absent in both parents. RAPD markers presenting in both parents were the major source of non-segregation markers; markers presenting in only one parent mainly showed Mendelian segregation. There were five non-parental markers. Markers showing 1: 1 and 3: 1 segregation were used for constructing an RAPD molecular linkage map. The results demonstrated that RAPD markers could be used to study the genomes of the wild grapes native to China. (C) 2002 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据