期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 77, 期 5, 页码 489-494出版社
JOHN WILEY & SONS INC
DOI: 10.1002/bit.10177
关键词
sophorose lipids; Candida bombicola; oxygen transfer rate; optimal aeration; fed-batch culture; lactonic form; acidic form
Sophorose lipids (SLs) have applications as surfactants and are produced at high levels by several yeasts. We developed a fed-batch shake-flask method for the production of SLs by Candida bombicola ATCC 22214. Optimal aeration, expressed in terms of oxygen transfer rate, was between 50 and 80 mM O-2/L h(-1) and resulted in maximum values for both volumetric product formation (1-1.5 g/L h(-1)) and SL yield (350 g/L). The lowest aeration levels resulted in the enrichment in saturated fatty acid SLs at the expense of unsaturated fatty acid SLs. (C) 2002 John Wiley Sons, Inc.
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