期刊
FEBS LETTERS
卷 514, 期 1, 页码 37-43出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(02)02328-1
关键词
unusual tRNA; mitochondrion; nematode; nuclear magnetic resonance; stable isotope; Ascaris suum
The tertiary structure of Ascaris suum mitochondrial tRNA(VCU)(Ser) was examined by nuclear magnetic resonance analysis using its transcript, since tRNA(UCU)(Ser), lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNA(UCU)(Ser) were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3'-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNA(UCU)(Ser) appears to function in a similar way to that of usual tRNAs in the ribosome. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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